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- Title
Microorganism viability influences internal phase droplet size changes during storage in water-in-oil emulsions.
- Authors
VanderGheynst, Jean; Guo, Hong-Yun; Cheng, Yu-Shen; Scher, Herbert
- Abstract
Water-in-oil emulsions provide an alternative for long-term stabilization of microorganisms. Maintaining physical stability of the emulsion and cell viability is critical for large-scale application. Water-in-oil (W/O) emulsions were prepared with the biolarvacide Lagenidium giganteum and the green alga Chlorella vulgaris. Physical stability was measured via light scattering measurements of the internal phase droplets and cell viability was measured by plating and enumerating colony forming units. Emulsions were demonstrated to stabilize L. giganteum and C. vulgaris for more than 4 months without refrigeration. Introducing nutrients into the internal phase of W/O emulsions without cells had no significant effect on changes in aqueous phase droplet size dynamics. Internal phase droplet size changes that occurred over time were greater in the presence of cells. Increases in droplet size were correlated with cell death indicating measurement of internal phase droplet size changes may be an approach for monitoring declines in cell viability during storage.
- Subjects
EMULSIONS; COMPOSITION of microorganisms; LAGENIDIUM; CHLORELLA vulgaris; CELL death
- Publication
Bioprocess & Biosystems Engineering, 2013, Vol 36, Issue 10, p1427
- ISSN
1615-7591
- Publication type
Article
- DOI
10.1007/s00449-013-0886-6