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- Title
Improved enzymatic performance of graphene‐immobilized β‐glucosidase A in the presence of glucose‐6‐phosphate.
- Authors
Albino Gomes, Anderson; Pazinatto Telli, Elisa; Miletti, Luiz Claudio; Skoronski, Everton; Gomes Ghislandi, Marcos; Felippe da Silva, Gustavo; Borba Magalhães, Maria de Lourdes
- Abstract
Abstract: Optimization of cellulose enzymatic hydrolysis is crucial for cost‐effective bioethanol production from lignocellulosic biomass. Enzyme immobilization in solid support allows enzyme recycling for reuse, lowering hydrolysis costs. Graphene is a nanomaterial isolated in 2004, which possesses exceptional properties for biomolecule immobilization. This study evaluates the potential for β‐glucosidase recycling by immobilization on graphene nanosheets. Data reported here demonstrated that graphene‐immobilized β‐glucosidase can be recycled for at least eight cycles. Immobilization did not change the optimal temperature of catalysis and improved enzymatic stability upon storage. The role of glucose‐6‐phosphate on immobilized enzyme was also investigated, demonstrating that glucose‐6‐phosphate acts as a mixed‐type activator and improves storage stability of immobilized enzyme. Complete cellulose hydrolysis using graphene‐immobilized β‐glucosidase in the presence of glucose‐6‐phosphate resulted in greatly improved hydrolysis rates, demonstrating the potential of this strategy for biomass hydrolysis.
- Subjects
ETHANOL as fuel; BETA-glucosidase; BACILLUS polymyxa; ENZYMES; ENCAPSULATION (Catalysis)
- Publication
Biotechnology & Applied Biochemistry, 2018, Vol 65, Issue 2, p246
- ISSN
0885-4513
- Publication type
Article
- DOI
10.1002/bab.1569