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- Title
Study of the crystal shape and its influence on the antitumor activity of tumor necrosis factorrelated apoptosisinducing ligand Apo2LTRAIL.
- Authors
JiCheng Yin; JinSong Zhou; Jing Sun; Yi Qiu; DongZhi Wei
- Abstract
This paper describes a method about the crystal shape control improving the antitumor activity of tumor necrosis factorrelated apoptosisinducing ligand Apo2LTRAIL in a batch cooling suspension crystallization by selecting pH values as a controllable variable. Three shaped TRAIL crystals could be obtained under different pH conditions, among which the hexagonal plate crystals had the highest specific activities against tumor cell line. The relationship among pH values, trimer contents and the specific activities of crystals and the purified TRAIL solutions were investigated. The results showed that different trimer contents resulted from pH altering in crystals and protein solutions is a main reason for their different specific activities. The studies may supply a new method to improve the bioactivity of TRAIL agent during its production and storage. © 2008 WILEYVCH Verlag GmbH & Co. KGaA, Weinheim
- Subjects
CRYSTALLOGRAPHY; PHYSICAL sciences; ANISOTROPY; ASTERISM (Crystallography)
- Publication
Crystal Research & Technology, 2008, Vol 43, Issue 8, p888
- ISSN
0232-1300
- Publication type
Article
- DOI
10.1002/crat.200711135