We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
鞭毛蛋白诱导表达差异基因的分析.
- Authors
李 双; 杨泽敏; 廖义潇; 杨 颖
- Abstract
The study was to analyze the characteristics of gene expression profiles stimulated by flagellin adjuvants, to reveal potential mechanism of flagellin adjuvant. The target microarray datasets GSE46421 and GSE72016 were screened from GEO database, and DEGs of two datasets were analyzed. The GO biological functions and KEGG signaling pathway involved in DEGs were analyzed by DAVID, PPI network of DEGs was constructed by String and visualized by Cytoscape. PPI network was visualized by Cytoscape, PPI sub-modules and hub genes with high connectivity were screened. The results showed that a total of 182 DEGs were screened, including 161 up-regulated genes and 121 downregulated genes. DEGs involved in GO biological processes mainly included signal transduction, immune response, inflammatory response, apoptosis, etc., molecular functions include kinase activation, receptor binding, cytokine activation and membrane signal transduction. The DEGs involved in KEGG pathways included cytokine-cytokine receptor interaction, TNF signaling pathway, NF-κB signaling pathway, Toll-like receptor signaling pathway, NOD-like receptor signaling pathway and other immune-related pathways. Analysis of the PPI network of DEGs revealed two important PPI sub-modules and ten key genes (IL-10, IL-6, CCL2, CXCL2, NFKBIA, MyD88, etc.). The study indicates that flagellin triggers MyD88-dependent and MyD88-independent pathways to signal and activate transcription factor NF- κB by recognizing TLR5 and NOD-like receptors. These signals activate the immune system and induce the production of various cytokines and chemokines in body through a cascade reaction, which plays an adjuvant effect.
- Subjects
GENE expression profiling; TRANSCRIPTION factors; CELLULAR signal transduction; FLAGELLIN; TOLL-like receptors; CYTOKINE receptors; CHEMOKINE receptors
- Publication
Feed Research, 2023, Vol 46, Issue 4, p69
- ISSN
1002-2813
- Publication type
Article
- DOI
10.13557/j.cnki.issn1002-2813.2023.04.015