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- Title
MicroRNA-141-3p Negatively Modulates SDF-1 Expression in Age-Dependent Pathophysiology of Human and Murine Bone Marrow Stromal Cells.
- Authors
Periyasamy-Thandavan, Sudharsan; Burke, John; Mendhe, Bharati; Kondrikova, Galina; Kolhe, Ravindra; Hunter, Monte; Isales, Carlos M; Hamrick, Mark W; Hill, William D; Fulzele, Sadanand
- Abstract
Stromal cell-derived factor-1 (SDF-1 or CXCL12) is a cytokine secreted by cells including bone marrow stromal cells (BMSCs). SDF-1 plays a vital role in BMSC migration, survival, and differentiation. Our group previously reported the role of SDF-1 in osteogenic differentiation in vitro and bone formation in vivo; however, our understanding of the post-transcriptional regulatory mechanism of SDF-1 remains poor. MicroRNAs are small noncoding RNAs that post-transcriptionally regulate the messenger RNAs (mRNAs) of protein-coding genes. In this study, we aimed to investigate the impact of miR-141-3p on SDF-1 expression in BMSCs and its importance in the aging bone marrow (BM) microenvironment. Our data demonstrated that murine and human BMSCs expressed miR-141-3p that repressed SDF-1 gene expression at the functional level (luciferase reporter assay) by targeting the 3'-untranslated region of mRNA. We also found that transfection of miR-141-3p decreased osteogenic markers in human BMSCs. Our results demonstrate that miR-141-3p expression increases with age, while SDF-1 decreases in both the human and mouse BM niche. Taken together, these results support that miR-141-3p is a novel regulator of SDF-1 in bone cells and plays an important role in the age-dependent pathophysiology of murine and human BM niche.
- Subjects
MESENCHYMAL stem cells; BONE cells; PATHOLOGICAL physiology; MESSENGER RNA; NON-coding RNA
- Publication
Journals of Gerontology Series A: Biological Sciences & Medical Sciences, 2019, Vol 74, Issue 9, p1368
- ISSN
1079-5006
- Publication type
journal article
- DOI
10.1093/gerona/gly186