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- Title
Hydrolysate of highly digestible phosphoryiated buckwheat major allergen Fag e 2 attenuates allergic reactions in Fag e 2-sensitized mice.
- Authors
Al Athamneh, Ahmad M.; Yuta Suzuki; Soichiro Nakamura; Shigeru Katayama
- Abstract
Fag e 2 is a 16 kDa major buckwheat allergen that is highly resistant to pepsin digestion. We previously reported that phosphorylation reduced the IgE-binding activity of Fag e 2 and oral administration of phosphoryiated Fag e 2 (P-Fag e 2) attenuated allergic reactions in mice. In this study, we investigated the effects of phosphorylation on the digestibility of Fag e 2 and assessed whether digested P-Fag e 2 (DP-Fag e 2) can attenuate allergic reactions in Fag e 2-sensitized mice. Recombinant Fag e 2, obtained using the Pichia expression system,was phosphoryiated via dry-heating in the presence of pyrophosphate. The peptic digestibility of Fag e 2 was enhanced by phosphorylation. Mice orally administered DP-Fag e 2 for 6 weeks after Fag e 2 sensitization exhibited reduced allergic symptom scores compared to those of sham-treated mice. Furthermore, decreased total and specific IgE, decreased specific IgGi,and increased total IgA were observed in the serum of the DP-Fag e 2-fed group. These results suggest that P-Fag e 2 is easily digested in the stomach and induces the attenuation of the IgE-mediated allergic reaction.
- Subjects
BUCKWHEAT; ALLERGENS; PHOSPHORYLATION; ALLERGIES; PYROPHOSPHATES
- Publication
Japanese Journal of Food Chemistry & Safety, 2020, Vol 27, Issue 2, p67
- ISSN
1341-2094
- Publication type
Article