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- Title
高温蛋白质降解菌的筛选及其在鸡粪发酵中的应用.
- Authors
袁小懿; 梁钰灵; 蒋 敏; 郭明月; 徐爱玲
- Abstract
This study aims to lay the foundation for the rational use of chicken manure resources and the production of high-quality organic fertilizers through the enrichment cultivation, screening and separation of high-temperature protein degrading bacteria. Due to the lack of protein-degrading bacteria and high-temperature bacteria in the process of chicken manure fermentation, the fermentation heating time is slow, the high temperature maintenance time is short, and the fermentation time is long. Enriched culture, purification and separation of high-temperature strains that efficiently degrade proteins under high-temperature conditions. 24 strains of high-temperature and highly efficient protein degradation bacteria were isolated and identified as belonging to 8 species, including Brevibacillus spp, Bacillus spp, and Pseudoxanthomonas spp. Inoculation of the prepared compound bacterial solution in chicken manure found that the the temperature rise rate in the experimental group with the bacterial agent was significantly faster than that in the control group. It reached the high temperature stage about 16h after fermentation, nearly 8h ahead of the blank group, and which was nearly 8 h earlier than that in the control group. And the FJ3 group, which was added with high-temperature protein-degrading bacteria. Compared with that in the control group, the high temperature period was prolonged by about 24 hours, and the duration reached 56 hours. The pH of the fermentation process decreased faster than that of the control group, the lowest pH value was around 5.5. The moisture content of the poultry dung reduced from 65% to about 20%, the proportion of N, P, K and other nutrients increased, and the fermentation product is loose and soft, with a strong acid flavor. Therefore, the addition of high-temperature protein-degrading bacteria could increase the temperature rise rate, prolong the period of high temperature, accelerate the ferment process of poultry dung, and reduce the loss of nutrients, which has great significance for the treatment of poultry dung.
- Subjects
POULTRY manure; ORGANIC fertilizers; POULTRY processing; BACTERIAL proteins; PROTEOLYSIS; BACILLUS amyloliquefaciens
- Publication
Progress in Modern Biomedicine, 2020, Vol 20, Issue 16, p3043
- ISSN
1673-6273
- Publication type
Article
- DOI
10.13241/j.cnki.pmb.2020.16.009