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- Title
下丘脑催产素对大鼠摄食和胃动力的影响及调控研究.
- Authors
刘湛; 王咪; 孙向荣; 高胜利; 郭菲菲; 徐珞
- Abstract
To investigate the effects of hypothalamic oxytocin (OXT) on the feeding and gastric motility in rats and its regulatory mechanism. The neural pathway between the supraoptic nucleus (SON) and arcuate nucleus (ARC) was observed by fluorescent gold retrograde tracing combined with immunohistochemical experiments; The effects of ARC microinjection of OXT on feeding in rats was observed by brain nuclei catheterization; The effects of electrical stimulation of SON on gastric motility in rats was observed by unipolar electrical stimulation; The effects of ARC microinjection of OXT on gastric motility and gastric emptying in rats was observed. Fluorescent gold retrograde tracing combined with immunohistochemical experiments showed that there was a neural pathway between SON and ARC in rats; After microinjection of OXT into ARC, the food intake of rats decreased significantly at 0-2h, 0-3h and 0-4h. Atosiban, an OXT receptor antagonist, completely blocked the inhibitory effect of OXT. After injection of mixture of OXT and MK-329, a cholecystokinin (CCK) receptor antagonist, the inhibitory effect of OXT on feeding in rats was partially blocked; Electrical stimulation of SON increased the amplitude and frequency of gastric motility in rats, and the effects of electrical stimulation of SON was further enhanced after microinjection of atosiban into ARC; After microinjection of OXT into ARC, the amplitude and frequency of gastric motility in rats decreased significantly. Atosiban completely blocked the inhibition of OXT on the amplitude and frequency of gastric motility. MK-329 partially blocked the inhibition of OXT on the amplitude and frequency of gastric motility; After microinjection of OTX into ARC, the gastric emptying rate of rats decreased significantly. Atosiban completely blocked the inhibition of OXT on gastric emptying, while MK-329 partially blocked the inhibition of OXT on gastric emptying. SON-ARC has a OXT neural pathway, which is mediated by the CCK.
- Subjects
GASTROINTESTINAL motility; GASTRIC emptying; NEURAL pathways; INGESTION; MICROINJECTIONS
- Publication
Progress in Modern Biomedicine, 2019, Vol 19, Issue 17, p3244
- ISSN
1673-6273
- Publication type
Article
- DOI
10.13241/j.cnki.pmb.2019.17.009