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- Title
Ghrelin 对大鼠摄食的影响及orexins信号通路的调控.
- Authors
陈雪焕; 王程; 郭菲菲; 孙向荣; 徐珞
- Abstract
Objective: To investigate the effect of ghrelin on the feeding of rats and the regulation of orexins signaling pathway. Methods: Ininiunohistochenncal staining was used to observe the synaptic connections of ghrelin lnimunoreactive neurons axon terminals and orexin-producing and the expression of c-fos in hypothalamic lateral area (LHA). After intracerebroventricular injection of anti-orex-in-A IgG and anti-orexin-B IgG. anti-melanin-concentrating hormone (MCH) IgG. NPY-1 receptor antagonist, food intake was measured to observe its effect on ghrelin-induced food intake. Results: Ghrelin-immunoreactive axonal terminals made direct synaptic contacts with orexin-producing neurons. Intracerebroventricular administration of ghrelin induced c-fos expression in orexin-producing neurons but not in MCH-producing neurons. Pre-injection of anti-NPY IgG antibody, ghrelin still induced c-fos expression in orexin-producing neurons. Pretreatment with anti-orexin-A IgG and anti-orexin-B IgG. but not anti-MCH IgG. attenuated ghrelin-induced feeding. Administration of NPY receptor antagonist further attenuated ghrelin-induced feeding in rats treated with anti-orexinA-IgG and anti-orexinB-IgG. Conclusion: ghrelin may interact with the orexins system to participate in the regulation of feeding behavior and energy homeostasis.
- Publication
Progress in Modern Biomedicine, 2018, Vol 18, Issue 12, p2248
- ISSN
1673-6273
- Publication type
Article
- DOI
10.13241/j.cnki.pmb.2018.12.010