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- Title
改良的乳鼠原代心房肌细胞的培养及鉴定方法.
- Authors
闫东霞; 趙越; 李耕慧; 张丽丽
- Abstract
Objective: To investigate the culture and identification of major primary atrial myocytes, which can be used to study the remodelling of atrium or relative future research. Methods: Atrial and ventricular muscle is isolated from 40 Sprague Dawley rats(1-3days old, male or female), trypsin EDTA is added to digest fully, then atrial myocytes were purified with differential adhesion method and passage and identified with immunocytochemistry. Results: Atrial myocytes coverage are up to 90% after 72 hours primary culture, and show the feature of volatility, 90% of myocytes of atrium can be authenticated by α-actin antibody staining with immunocytochemistry.Conclusion: Atrial myocytes with high purity and vigor can be separated by enzyme digestion, it is a better method of cultivation and identification of atrial myocytes of neonatal rat.
- Publication
Progress in Modern Biomedicine, 2016, Vol 16, Issue 3, p442
- ISSN
1673-6273
- Publication type
Article
- DOI
10.13241/j.cnki.pmb.2016.03.010