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- Title
Orexin-A 介导 histamine 能神经系统促进氯胺酮麻醉觉醒.
- Authors
王志华; 邹丽丽; 张巧梅; 吴畏; 李健楠; 张丽娜; 孟尽海
- Abstract
Objective: The aim of this study was to test whether activation of orexin signal in Tuberomammillary Nucleus(TMN)can facilitate emergence from katemine anesthesia or not. Methods: Adult male SD rats(body weight 230-280 g) were anesthetized by10% chloral hydrate(1 ml/kg, ip) to do the following work. 1Guide cannulas for microinjection to the TMN were embedded. Then the rats were fed in separate cage. Seven days later, rats were randomly divided into three groups. And then orexin-A(100 pmol/0.3 μL),orexin-B(100 pmol/0.3 μL) and and their solvent NS(0.3 μL) were microinjected into TMN respectively to observe the induction time and awakening time in rats. 2 The same operation as step 1, Seven days later, rats were grouped randomly. And then the OX1 R antagonist SB334867(20 μL/0.3 μL), OX2 R antagonist TCS-OX2-29 and their solvent DMSO(0.3 μL) were microinjected into TMN respectively to observe the time of induction and awakening in rats. Results: 1No statistical difference of induction time was found in each group. 2The rats which injected orexin-A(100 pmol/0.3 μL) recovered much faster from ketamine anesthesia(100 mg/kg, ip) than controlled group(injected NS 0.3 μL)(43.17 ±6.31 min vs 51.17 ±4.45 min,P<0.05).There was no significant difference on the emergence from ketamine anesthesia between the rats received orexin-B and controlled rats(50.33 ±3.50 min vs 51.17 ±4.45 min,P>0.05). 3 The injection of OX1 R antagonist SB334867(20 μL/0.3 μL) to TMN could prolong the emergence time from ketamine anesthesia compared to the solvent DMSO(0.3 μL) group(60.83 ±8.84 min vs 49.00 ±5.73 min, P<0.05). The injection of OX2 R antagonist TCS-OX2-29 did not significantly affect the rat emergence time(50.83±4.79 min vs 49.00±5.73 min, P>0.05). Conclusion:Orexin-A Can facilitates emergence awakening from ketamine anesthesia through histaminergic system in TMN.
- Publication
Progress in Modern Biomedicine, 2015, Vol 15, Issue 14, p2663
- ISSN
1673-6273
- Publication type
Article
- DOI
10.13241/j.cnki.pmb.2015.14.016