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- Title
Mutations in eIF5B Confer Thermosensitive and Pleiotropic Phenotypes via Translation Defects in Arabidopsis thaliana.
- Authors
Zhang, Liyuan; Liu, Xinye; Gaikwad, Kishor; Kou, Xiaoxia; Wang, Fei; Tian, Xuejun; Xin, Mingming; Ni, Zhongfu; Sun, Qixin; Peng, Huiru; Vierling, Elizabeth
- Abstract
The conserved eukaryotic translation initiation factor 5B, eIF5B, is a GTPase that acts late in translation initiation. We found that an Arabidopsis thaliana mutant sensitive to hot temperatures 3 (hot3-1), which behaves as the wild type in the absence of stress but is unable to acclimate to high temperature, carries a missense mutation in the eIF5B1 gene (At1g76810), producing a temperature sensitive protein. A more severe, T-DNA insertion allele (hot3-2) causes pleiotropic developmental phenotypes. Surprisingly, Arabidopsis has three other eIF5B genes that do not substitute for eIF5B1 ; two of these appear to be in the process of pseudogenization. Polysome profiling and RNA-seq analysis of hot3-1 plants show delayed recovery of polysomes after heat stress and reduced translational efficiency (TE) of a subset of stress protective proteins, demonstrating the critical role of translational control early in heat acclimation. Plants carrying the severe hot3-2 allele show decreased TE of auxin-regulated, ribosome-related, and electron transport genes, even under optimal growth conditions. The hot3-2 data suggest that disrupting specific eIF5B interactions on the ribosome can, directly or indirectly, differentially affect translation. Thus, modulating eIF5B interactions could be another mechanism of gene-specific translational control.
- Subjects
PHENOTYPES; HEAT shock proteins; MISSENSE mutation; ELECTRON transport; GENETIC translation; ACCLIMATIZATION; GENETIC mutation
- Publication
Plant Cell, 2017, Vol 29, Issue 8, p1952
- ISSN
1040-4651
- Publication type
Article
- DOI
10.1105/tpc.16.00808