We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Removal of Phenol by Rhodococcus opacus 1CP after Dormancy: Insight into Enzymes' Induction, Specificity, and Cells Viability.
- Authors
Egozarian, Natalia S.; Emelyanova, Elena V.; Suzina, Nataliya E.; Sazonova, Olesya I.; Polivtseva, Valentina N.; Anokhina, Tatiana O.; Wu, Yonghong; Solyanikova, Inna P.
- Abstract
Biodegradation of phenol is an effective method for removing this toxicant from contaminated sites. Phenol is a toxic compound for living cells, so many bacteria degrade phenol in relatively low concentrations, up to 0.75 g L−1. The Rhodococcus opacus strain 1CP is an effective destructor of a wide range of pollutants. In the absence of a carbon source in the medium, cells of the R. opacus 1CP strain easily form cyst-like resting cells (CLC). The purpose of this work was to evaluate the viability of cells during long-term storage and the efficiency of the process of phenol destruction by R. opacus 1CP cells germinating after dormancy. Resting cells were obtained by simple cultivation in a rich medium followed by storage under static conditions. This is a simple approach to obtain a large amount of biomass. Decomposition of phenol proceeded via catechol followed by ortho-cleavage of aromatic ring. The induction of three phenol hydroxylases was detected by RT-PCR in cells germinated in a mineral medium with phenol as the carbon source. The stability of the genome of cells germinating after dormancy is shown by box-PCR. Dormant R. opacus 1CP cells, both suspended and immobilized, can be directly used for the decomposition of phenol after 4–12 months storage. In addition to phenol, after 9 months of storage, immobilized germinating cells easily metabolized 4-chlorophenol and 2,4,6-trichlorophenol. The results demonstrate a potential and simple approach toward achieving long-term storage of cells for further use in bioremediation.
- Subjects
CELL survival; PHENOL; RHODOCOCCUS; HAZARDOUS waste sites; IMMOBILIZED cells; TRICHLOROPHENOL; CATECHOL
- Publication
Microorganisms, 2024, Vol 12, Issue 3, p597
- ISSN
2076-2607
- Publication type
Article
- DOI
10.3390/microorganisms12030597