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- Title
Enzymatic Preparation of 2,5-Furandicarboxylic Acid (FDCA)--A Substitute of Terephthalic Acid--By the Joined Action of Three Fungal Enzymes.
- Authors
Karich, Alexander; Kleeberg, Sebastian B.; Ullrich, René; Hofrichter, Martin
- Abstract
Enzymatic oxidation of 5-hydroxymethylfurfural (HMF) and its oxidized derivatives was studied using three fungal enzymes: wild-type aryl alcohol oxidase (AAO) from three fungal species, wild-type peroxygenase from Agrocybe aegerita (AaeUPO), and recombinant galactose oxidase (GAO). The effect of pH on different reaction steps was evaluated and apparent kinetic data (Michaelis-Menten constants, turnover numbers, specific constants) were calculated for different enzyme-substrate ratios and enzyme combinations. Finally, the target product, 2,5-furandicarboxylic acid (FDCA), was prepared in a multi-enzyme cascade reaction combining three fungal oxidoreductases at micro-scale. Furthermore, an oxidase-like reaction is proposed for heme-containing peroxidases, such as UPO, horseradish peroxidase, or catalase, causing the conversion of 5-formyl-2-furancarboxylic acid into FDCA in the absence of exogenous hydrogen peroxide.
- Subjects
FUNGAL enzymes; TEREPHTHALIC acid; ENZYMATIC analysis; ALCOHOL oxidase; CHEMICAL reactions
- Publication
Microorganisms, 2018, Vol 6, Issue 1, p5
- ISSN
2076-2607
- Publication type
Article
- DOI
10.3390/microorganisms6010005