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- Title
Chromatin Immunoprecipitation Reveals the 180-bp Satellite Repeat Is the Key Functional DNA Element of Arabidopsis thaliana Centromeres.
- Authors
Nagaki, Kiyotaka; Talbert, Paul B.; Zhong, Cathy Xiaoyan; Dawe, R. Kelly; Henikoff, Steven; Jiang, Jiming
- Abstract
The centromeres of Arabidopsis thaliana chromosomes contain megabases of complex DNA consisting of numerous types of repetitive DNA elements. We developed a chromatin immunoprecipitation (CHIP) technique using an antibody against the centromeric H3 histone, HTR12, in Arabidopsis. ChIP assays showed that the 180-bp centromeric satellite repeat was precipitated with the antibody, suggesting that this repeat is the key component of the centromere/kinetochore complex in Arabidopsis.
- Subjects
CENTROMERE; PLANT chromosomes; CHROMATIN; MICROSATELLITE repeats; ARABIDOPSIS
- Publication
Genetics, 2003, Vol 163, Issue 3, p1221
- ISSN
0016-6731
- Publication type
Article
- DOI
10.1093/genetics/163.3.1221