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- Title
Search for genes influencing the maintenance of the [ ISP<sup>+</sup>] prion-like antisuppressor determinant in yeast with the use of an insertion gene library.
- Authors
Rogoza, T. M.; Viktorovskaya, O. V.; Rodionova, S. A.; Ivanov, M. S.; Volkov, K. V.; Mironova, L. N.
- Abstract
The prion-like determinant [ ISP+] manifests itself as an antisuppressor of certain sup35 mutations. To establish that [ ISP+] is indeed a new yeast prion, it is necessary to identify the gene that codes for the protein whose prion form is [ ISP+]. Analysis of the transformants obtained by transformation of an [ ISP+] strain with an insertion gene library revealed three genes controlling the [ ISP+] maintenance: UPF1, UPF2, and SFP1. SFP1 codes for a potentially prionogenic protein, which is enriched in Asn and Gln residues, and is thereby the most likely candidate for the [ ISP+] structural gene. UPF1 and UPF2 code for components of nonsense-mediated mRNA decay. The [ ISP+] elimination caused by UPF1 and UPF2 inactivation was reversible, and Upf1p and Upf2p were not functionally related to phosphatase Ppz1p, which influences the [ ISP+] manifestation. Possible mechanisms sustaining the influence of UPF1 and UPF2 on [ ISP+] maintenance are discussed.
- Subjects
PRIONS; NONSENSE suppression (Genetics); YEAST fungi genetics; MESSENGER RNA; PHOSPHATASES
- Publication
Molecular Biology, 2009, Vol 43, Issue 3, p360
- ISSN
0026-8933
- Publication type
Article
- DOI
10.1134/S0026893309030029