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- Title
Molecular Mechanisms of Translation Initiation in Mammals Studied by in vitroReconstruction of Initiation Complexes.
- Authors
Shatsky, I. N.
- Abstract
Papers on the mechanisms of translation initiation in mammals studied by reconstruction of initiation complexes from individual components are reviewed. The author points to the constraints of this approach and to the pitfalls ignoring which one might come to erroneous conclusions and even artifacts. In addition, some methods employed in the field as well as some technical problems are discussed in the paper, together with the means of obviating them. The review could be a guidebook for newcomers into this quite labor-consuming field.
- Subjects
MOLECULES; MAMMALS; VERTEBRATES; AVOGADRO'S law; BIOMOLECULES; ANTIQUITIES
- Publication
Molecular Biology, 2001, Vol 35, Issue 4, p536
- ISSN
0026-8933
- Publication type
Article
- DOI
10.1023/A:1010566808784