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- Title
Involvement of the flagellar assembly pathway in Vibrio alginolyticus adhesion under environmental stresses.
- Authors
Lu Wang; Lixing Huang; Yongquan Su; Yingxue Qin; Wendi Kong; Ying Ma; Xiaojin Xu; Mao Lin; Jiang Zheng; Qingpi Yan
- Abstract
Adhesion is an important virulence factor of Vibrio alginolyticus. This factor may be affected by environmental conditions; however, its molecular mechanism remains unclear. In our previous research, adhesion deficient strains were obtained by culturing V. alginolyticus under stresses including Cu, Pb, Hg, and low pH. With RNA-seq and bioinformatics analysis, we found that all of these stress treatments significantly affected the flagellar assembly pathway, which may play an important role in V. alginolyticus adhesion. Therefore, we hypothesized that the environmental stresses of the flagellar assembly pathway may be one way in which environmental conditions affect adhesion. To verify our hypothesis, a bioinformatics analysis, QPCR, RNAi, in vitro adhesion assay and motility assay were performed. Our results indicated that (1) the flagellar assembly pathway was sensitive to environmental stresses, (2) the flagellar assembly pathway played an important role in V. alginolyticus adhesion, and (3) motility is not the only way in which the flagellar assembly pathway affects adhesion.
- Subjects
VIBRIONACEAE; ADSORPTION (Chemistry); VIBRIO alginolyticus; ENVIRONMENTAL engineering; ADHESION
- Publication
Frontiers in Cellular & Infection Microbiology, 2015, p1
- ISSN
2235-2988
- Publication type
Article
- DOI
10.3389/fcimb.2015.00059