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- Title
Spontaneous Electrical Activity of Cultured Interstitial Cells of Cajal from Mouse Urinary Bladder.
- Authors
Sun-Ouck Kim; Han-Seong Jeong; Sujeong Jang; Mei-Jin Wu; Jong Kyu Park; Han-Yi Jiao; Jae Yeoul Jun; Jong-Seong Park
- Abstract
Interstitial cells of Cajal (ICCs) from the urinary bladder regulate detrusor smooth muscle activities. We cultured ICCs from the urinary bladder of mice and performed patch clamp and intracellular Ca2+ ([Ca2+]i) imaging to investigate whether cultured ICCs can be a valuable tool for cellular functional studies. The cultured ICCs displayed two types of spontaneous electrical activities which are similar to those recorded in intact bladder tissues. Spontaneous electrical activities of cultured ICCs were nifedipine-sensitive. Carbachol and ATP, both excitatory neurotransmitters in the urinary bladder, depolarized the membrane and increased the frequency of spike potentials. Carbachol increased [Ca2+]i oscillations and basal Ca2+ levels, which were blocked by atropine. These results suggest that cultured ICCs from the urinary bladder retain rhythmic phenotypes similar to the spontaneous electrical activities recorded from the intact urinary bladder. Therefore, we suggest that cultured ICCs from the urinary bladder may be useful for cellular and molecular studies of ICCs.
- Subjects
BLADDER; COILED bodies (Cytology); INTERSTITIAL cells; NEUROTRANSMITTERS; ACTION potentials; CALCIUM ions
- Publication
Korean Journal of Physiology & Pharmacology, 2013, Vol 17, Issue 6, p531
- ISSN
1226-4512
- Publication type
Article
- DOI
10.4196/kjpp.2013.17.6.531