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- Title
Test of the paired-flash electroretinographic method in mice lacking b-waves.
- Authors
JENNIFER J. KANG DERWENT; SHANNON M. SASZIK; HIDETAKA MAEDA; DEBORAH M. LITTLE; MACHELLE T. PARDUE; LAURA J. FRISHMAN; DAVID R. PEPPERBERG
- Abstract
Previous studies of rod photoreceptors in vivohave employed a paired-flash electroretinographic (ERG) technique to determine rod response properties. To test whether absence versuspresence of the ERG b-wave affects the photoreceptor response derived by the paired-flash method, we examined paired-flash-derived responses obtained from nobmice, a mutant strain with a defect in signal transduction between photoreceptors and ON bipolar cells that causes a lack of the b-wave. Normal littermates of the nobmice served as controls. The normalized amplitude-intensity relation of the derived response determined in nobmice at the near-peak time of 86 ms was similar to that determined for the controls. The full time course of the derived rod response was obtained for test flash strengths ranging from 0.11 to 17.38 scotopic cd s m−2(sc cd s m−2). Time-course data obtained from noband control mice exhibited significant but generally modest differences. With saturating test flash strengths, half-recovery times for the derived response of nobversuscontrol mice differed by ∼60 ms or less about the combined (noband control) average respective values. Time course data also were obtained before versusafter intravitreal injection of l-2-amino-4-phosphonobutyrate (APB) (which blocks transmission from photoreceptors to depolarizing bipolar cells) and of cis2,3-piperidine dicarboxylic acid (PDA) (which blocks transmission to OFF bipolar cells, and to horizontal, amacrine and ganglion cells). Neither APB nor PDA substantially affected derived responses obtained from nobor control mice. The results provide quantitative information on the effect of b-wave removal on the paired-flash-derived response in mouse. They argue against a substantial skewing effect of the b-wave on the paired-flash-derived response obtained in normal mice and are consistent with the notion that, to good approximation, this derived response represents the isolated flash response of the photoreceptors in both noband normal mice.
- Subjects
PHOTORECEPTORS; BETA rhythm; ELECTRORETINOGRAPHY; EYE examination; MICE
- Publication
Visual Neuroscience, 2007, Vol 24, Issue 2, p141
- ISSN
0952-5238
- Publication type
Article
- DOI
10.1017/S0952523807070162