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- Title
Interaction of three-finger proteins from snake venoms and from mammalian brain with the cys-loop receptors and their models.
- Authors
Faure, G.; Shelukhina, I.; Porowinska, D.; Shulepko, M.; Lyukmanova, E.; Dolgikh, D.; Spirova, E.; Kasheverov, I.; Utkin, Yu.; Corringer, J.; Tsetlin, V.
- Abstract
With the use of surface plasmon resonance (SPR) it was shown that ws-Lynx1, a water-soluble analog of the three-finger membrane-bound protein Lynx1, that modulates the activity of brain nicotinic acetylcholine receptors (nAChRs), interacts with the acetylcholine-binding protein (AChBP) with high affinity, K = 62 nM. This result agrees with the earlier demonstrated competition of ws-Lynx1 with radioiodinated α-bungarotoxin for binding to AChBP. For the first time it was shown that ws-Lynx1 binds to GLIC, prokaryotic Cys-loop receptor ( K = 1.3 μM). On the contrary, SPR revealed that α-cobratoxin, a three-finger protein from cobra venom, does not bind to GLIC. Obtained results indicate that SPR is a promising method for analysis of topography of ws-Lynx1 binding sites using its mutants and those of AChBP and GLIC.
- Subjects
PROTEINS; SNAKE venom; MAMMALS; SURFACE plasmon resonance; NICOTINIC acetylcholine receptors; ACETYLCHOLINE-binding proteins; COBRA venom factor
- Publication
Doklady Biochemistry & Biophysics, 2016, Vol 468, Issue 1, p193
- ISSN
1607-6729
- Publication type
Article
- DOI
10.1134/S1607672916030091