We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
A new green approach to l-histidine and β-alanine analysis in dietary supplements using rapid and simple contactless conductivity detection integrated with high-resolution glass-microchip electrophoresis.
- Authors
Pukleš, Iva; Páger, Csilla; Sakač, Nikola; Matasović, Brunislav; Kovač-Andrić, Elvira; Šarkanj, Bojan; Samardžić, Mirela; Budetić, Mateja; Molnárová, Katarína; Marković, Dean; Vesinger, Ana; Jozanović, Marija
- Abstract
The analysis of dietary supplements is far less regulated than pharmaceuticals, leading to potential quality issues. Considering their positive effect, many athletes consume supplements containing l-histidine and β-alanine. A new microfluidic method for the determination of l-histidine and β-alanine in dietary supplement formulations has been developed. For the first time, capacitively coupled contactless conductivity detection was employed for the microchip electrophoresis of amino acids in real samples. A linear relationship between detector response and concentration was observed in the range of 10–100 µmol L–1 for l-histidine (R2 = 0.9968) and β-alanine (R2 = 0.9954), while achieved limits of detection (3 × S/N ratio) were 4.2 µmol L–1 and 5.2 µmol L–1, respectively. The accuracy of the method was confirmed using recovery experiments as well as CE-UV-VIS and HPLC-UV-VIS techniques. The developed method allows unambiguous identification of amino acids in native form without chemical derivatization and with the possibility of simultaneous analysis of amino acids with metal cations.
- Subjects
DIETARY supplements; AMINO acid analysis; ALANINE; HISTIDINE; MICROCHIP electrophoresis; ELEMENTAL diet; ELECTROPHORESIS
- Publication
Analytical & Bioanalytical Chemistry, 2024, Vol 416, Issue 15, p3605
- ISSN
1618-2642
- Publication type
Article
- DOI
10.1007/s00216-024-05314-9