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- Title
Transcriptional regulation of OCI-5/Glypican 3: elongation control of confluence-dependent induction.
- Authors
Li, M; Pullano, R; Yang, H L; Lee, H K; Miyamoto, N G; Filmus, J; Buick, R N
- Abstract
OCI-5/Glypican 3, a member of the glypican family of proteoglycans, is the defective gene in the Simpson-Golabi-Behmel overgrowth syndrome. OCI-5 expression is developmentally regulated in the intestinal epithelium, and the mechanism of its regulation was studied in the rat intestinal epithelial cell line IEC-18. A large induction of OCI-5 transcript and protein was observed at high cell density. Among other glypican family members, κ-glypican also exhibited a confluence-dependent induction in select cell types. Nuclear run-on analysis indicated that cell-density regulation of OCI-5 occurs at the level of transcription. The rat and mouse OCI-5 promoters were cloned and found to be highly conserved, located within CpG islands and contain regions of alternating purine and pyrimidine residues. No TATA-box or recognizable INR element was observed. Consensus binding sites for AP-2, SP-1, zeste and NF-1/CTF are conserved across human, mouse and rat promoters. 5′ deletion mapping of the rat promoter identified regions which enhance and repress promoter activity, with no apparent confluence-dependence or tissue-specificity. Nuclear run-on analysis probing different regions of the gene suggests that elongation control plays a role in the induction of OCI-5 by confluence.
- Subjects
PROTEOGLYCANS; INTESTINES; TRANSCRIPTION factors
- Publication
Oncogene, 1997, Vol 15, Issue 13, p1535
- ISSN
0950-9232
- Publication type
Article
- DOI
10.1038/sj.onc.1201325