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- Title
A novel technique to collect root exudates from mustard ( Brassica juncea).
- Authors
Wadhwa, Kunal; Narula, Neeru
- Abstract
A very simple, novel, cost effective, easy to use technique has been developed for the collection of root exudates from small seeded plants, under laboratory conditions. 200-1000 μl micro tips (Tarsons), kept in 100 ml glass beakers, were used as holders for the small seeds of Indian mustard ( Brassica juncea) and the exudates were trapped in liquid culture medium. The exudates, so obtained, were authenticated and analyzed for organic compounds such as sugars, amino acids and organic acids, as well as chemotactic response towards rhizobacteria. Method was found to be suitable and easy to handle for small seeds. (© 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)
- Subjects
PLANT exudates; EXUDATION (Botany); BRASSICA juncea; SEED microbiology; RHIZOBACTERIA; AMINO acids; ORGANIC acids
- Publication
Journal of Basic Microbiology, 2012, Vol 52, Issue 5, p613
- ISSN
0233-111X
- Publication type
Article
- DOI
10.1002/jobm.201100407