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- Title
SLC34A2 Targets in Calcium/Phosphorus Homeostasis of Mammary Gland and Involvement in Development of Clinical Mastitis in Dairy Cows.
- Authors
Wang, Xueying; Zhang, Bohao; Dong, Weitao; Zhao, Yu; Zhao, Xingxu; Zhang, Yong; Zhang, Quanwei
- Abstract
Simple Summary: Unbalanced Ca/P metabolism can induce mastitis in dairy cows, and solute carrier family 34 member 2 (SLC34A2) can affect transcellular inorganic phosphate absorption. In this study, the expression and localization of SLC34A2 in the mammary gland tissue of cows were detected and analyzed. In addition, based on the data-independent acquisition (DIA) proteomics data, we identified 12 critical differentially expressed proteins (DEPs) included in 11 Gene Ontology (GO) terms and two pathways that interacted with SLC34A2 using bioinformatics analysis. The results revealed that SLC34A2 and DEPs could regulate Ca/P metabolism and homeostasis and participate in the occurrence and development of clinical mastitis in dairy cows. These results provide a basis for understanding SLC34A2's function in Ca/P metabolism and the pathological mechanism of cow mastitis. The type II Na/Pi co-transporter (NaPi2b), encoded by the solute carrier (SLC) transporter 34A2 (SLC34A2), is responsible for calcium (Ca) and phosphorus (P) homeostasis. Unbalanced Ca/P metabolism induces mastitis in dairy cows. However, the specific role of SLC34A2 in regulating this imbalance in Holstein cows with clinical mastitis (CM) remains unclear. The aim of this study was to investigate the role of SLC34A2 and identify differentially expressed proteins (DEPs) that interact with SLC34A2 and are associated with Ca/P metabolism in dairy cows with CM. Immunohistochemical and immunofluorescence staining results showed that SLC34A2 was located primarily in the mammary epithelial cells of the mammary alveoli in both the control (healthy cows, Con/C) and CM groups. Compared to the Con/C group, the relative expression of the SLC34A2 gene and protein were significantly downregulated in the CM group. We identified 12 important DEPs included in 11 GO terms and two pathways interacting with SLC34A2 using data-independent acquisition proteomics. The PPI (protein-and-protein interaction) network results suggested that these DEPs were associated with ion metabolism and homeostasis, especially SLC34A2. These results demonstrate that SLC34A2 downregulation is negatively correlated with the occurrence and development of CM in Holstein cows, providing a basis for exploring the function and regulatory mechanism of SLC34A2 in Ca/P metabolism and homeostasis in Holstein cows with CM.
- Subjects
MASTITIS; DAIRY cattle; CALCIUM metabolism; MAMMARY glands; HOMEOSTASIS; IMMUNOSTAINING; CALCIUM
- Publication
Animals (2076-2615), 2024, Vol 14, Issue 9, p1275
- ISSN
2076-2615
- Publication type
Article
- DOI
10.3390/ani14091275