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- Title
Immune-Enhancing Effects of Limosilactobacillus fermentum in BALB/c Mice Immunosuppressed by Cyclophosphamide.
- Authors
Kim, SukJin; Lee, Hwan Hee; Kang, Chang-Ho; Kang, Hyojeung; Cho, Hyosun
- Abstract
This study evaluates the immune-enhancing effects of Limosilactobacillus fermentum on cyclophosphamide (CP)-induced immunosuppression in BALB/c mice. In vitro, the expressions of pro-inflammatory cytokines and MAPK signaling molecules in Raw264.7 cells were analyzed by ELISA and Western blot analysis. Moreover, cell proliferation, surface receptor expression, and cytotoxicity of NK-92 cells were examined by Cell Counting Kit-8, CytoTox96 assay, and flow cytometry, respectively. To investigate the immune-enhancing effects of selected L. fermentum strains in vivo, these strains were orally administered to BALB/c mice for 2 weeks, and CP was intraperitoneally injected. Then, liver, spleen, and whole blood were isolated from each animal. Administration of single L. fermentum strains or their mixture sustained the spleen weight, the counts of white blood cells compared to non-fed group. Splenocyte proliferation and NK cytotoxicity were significantly increased in all L. fermentum-fed groups. The frequency of B220+ cells was also significantly enhanced in splenocytes isolated from L. fermentum groups. In addition, the production of cytokines (TNF-α, IFN-γ) and antibodies was recovered in splenocyte supernatants isolated from L. fermentum groups. In conclusion, L. fermentum could be a suitable functional food additive for immune-enhancing effect.
- Subjects
IN vitro studies; CYTOKINES; FLOW cytometry; IN vivo studies; IMMUNOCOMPROMISED patients; ANIMAL experimentation; WESTERN immunoblotting; LIVER; CELL receptors; KILLER cells; INTRAPERITONEAL injections; TREATMENT effectiveness; GENE expression; CELLULAR signal transduction; IMMUNITY; CYCLOPHOSPHAMIDE; ENZYME-linked immunosorbent assay; CELL proliferation; TUMOR necrosis factors; DESCRIPTIVE statistics; LACTOBACILLUS; MITOGEN-activated protein kinases; SPLEEN; GRAM-positive bacteria; MICE
- Publication
Nutrients, 2023, Vol 15, Issue 4, p1038
- ISSN
2072-6643
- Publication type
Article
- DOI
10.3390/nu15041038