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- Title
Isolation, identification and toxinotyping of Clostridium perfringens isolated from broilers in Pakistan.
- Authors
Ul Abadeen, Zain; Javed, Muhammad Tariq; Rizvi, Farzana; Ur Rahman, Sajjad
- Abstract
Necrotic enteritis (NE) is one of the important enteric disease in the poultry industry worldwide, caused by C. perfringens type A. This study describes the isolation, identification, and toxinotyping of C. perfringens in necrotic enteritis affected broiler chicken in Pakistan. A total of 430 intestinal samples from dead carcasses and birds suspected of NE outbreak, in and around Faisalabad, Pakistan were collected from 36 broiler farms which yielded 87 alpha toxin gene (cpa) positive C. perfringens type A isolates. The birds having 4-5 weeks of age, clinical signs, and reared in open (conventional) sheds showed higher C. perfringens isolation rate. The study concluded netB negative C. perfringens type A as a causative agent for NE outbreaks in broiler birds in Faisalabad, Pakistan.
- Subjects
PAKISTAN; FAISALABAD (Pakistan); CLOSTRIDIUM perfringens; NECROTIC enteritis; POULTRY diseases; POULTRY farms; BROILER chickens; POULTRY industry
- Publication
Pakistan Journal of Agricultural Sciences, 2021, Vol 58, Issue 4, p1367
- ISSN
0552-9034
- Publication type
Article
- DOI
10.21162/PAKJAS/21.1414