We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Establishment of a highly efficient in vitro plant regeneration system for Impatiens hawkeri W. Bull cv. 'Pink' and its background resistance to kanamycin.
- Authors
Luo, Liang; Li, Yang; Huang, Xiaoyun; Zhang, Xiaoli; Tan, Yi; Liu, Zedong; Huang, Haiquan; Huang, Meijuan
- Abstract
New Guinea Impatiens (Impatiens hawkeri W. Bull) is a perennial succulent evergreen herb in the Balsaminaceae. This study primarily investigated the effects of different types of explants, seedling ages, and different plant growth regulators on the asexual propagation of New Guinea Impatiens and screened out its optimal concentration of kanamycin for genetic transformation. It provides a better method, theoretical conditions, and practical guidance for the rapid and highly efficient propagation of this plant and its background resistance to kanamycin. The cotyledon nodes of the New Guinea Impatiens that contained hypocotyls and cotyledons were used as experimental materials. The results showed that different types of explants had important effects on the highly efficient regeneration system of New Guinea Impatiens cv. 'Pink.' Murashige and Skoog (MS) media with 0.4 mg/L of 6-benzylaminopruine (6-BA) and 0.5 mg/L of thidiazuron (TDZ) were used to screen the best explants for the induction of adventitious shoots. The rate of induction was 93.33%, and the average number of adventitious buds was 18.87. MS media with 0.8 mg/L 6-BA + 1.0 mg/L TDZ + 0.1 mg/L indole-3-butyric acid (IBA) was the best medium for the proliferation of adventitious shoots among all the media tested. One-half MS + 0.1 mg/L IBA was superior to the other media for rooting, and the rooting rate reached 92.0%. All the explants died when kanamycin was administered at 75 mg/L. Thus, this was the background resistance for New Guinea Impatiens cv. 'Pink.' Key message: Different explant types, seedling ages, basic medium types, and hormone concentrations affect the efficiency of regeneration of New Guinea Impatiens cv. 'Pink.' The critical concentration of kanamycin was selected.
- Subjects
REGENERATION (Botany); IMPATIENS; KANAMYCIN; VEGETATIVE propagation; PLANT regulators
- Publication
Plant Cell, Tissue & Organ Culture, 2023, Vol 155, Issue 3, p627
- ISSN
0167-6857
- Publication type
Article
- DOI
10.1007/s11240-023-02580-w