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- Title
Preparation and in vitro tumor growth inhibitory effect of oligo (L-lactate) nanoparticles.
- Authors
Akihiro Matsumoto; Satoshi Murao; Chie Watanabe; Masahiro Murakami
- Abstract
Oligo L-lactates (oligolactates) that have low molecular weights less than 2000 have been reported to inhibit tumor growth and extend the survival of experimental animals. Because oligolactates are scarcely soluble in water, they require a solvent or a solubilizing agent, such as a surfactant, to be dissolved in water. However, these agents are generally cytotoxic, an in vitro assay appropriate to evaluate the inhibitory effect on tumor growth has not been developed yet. Here, we prepared a solid nanodispersion of oligolactates using an oil-in-water emulsion solvent evaporation method to evaluate its tumor inhibitory activity in vitro without a solvent or surfactant. Polyol solutions containing polyvinyl alcohol (PVA) were used as a continuous phase. The formation of nanoparticles depended on the concentrations of polyol and PVA in the continuous phase. The nanoparticles with a particle size of approximately 100 nm were obtained using 10-15% PVA and 60% propylene glycol. The obtained aqueous nanodispersion of oligolactates inhibited the growth of B16-BL6 melanoma cells in vitro, whereas the medium alone did not affect tumor cell growth. Therefore, oligo(L-lactate) nanoparticles may be useful in the research and development of oligolactates as a remedy for cancer.
- Subjects
TUMOR growth; NANOPARTICLE size; NANOPARTICLES; MOLECULAR weights; PARTICLES; PROPYLENE glycols; EMULSIONS (Pharmacy); POLYVINYL alcohol
- Publication
Drug Discoveries & Therapeutics, 2020, Vol 14, Issue 6, p296
- ISSN
1881-7831
- Publication type
Article
- DOI
10.5582/ddt.2020.03118