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- Title
Bone augmentation using a highly porous PLGA/β- TCP scaffold containing fibroblast growth factor-2.
- Authors
Yoshida, T.; Miyaji, H.; Otani, K.; Inoue, K.; Nakane, K.; Nishimura, H.; Ibara, A.; Shimada, A.; Ogawa, K.; Nishida, E.; Sugaya, T.; Sun, L.; Fugetsu, B.; Kawanami, M.
- Abstract
Background and Objective Beta-tricalcium phosphate (β- TCP), a bio-absorbable ceramic, facilitates bone conductivity. We constructed a highly porous three-dimensional scaffold, using β- TCP, for bone tissue engineering and coated it with co-poly lactic acid/glycolic acid ( PLGA) to improve the mechanical strength and biological performance. The aim of this study was to examine the effect of implantation of the PLGA/β- TCP scaffold loaded with fibroblast growth factor-2 ( FGF-2) on bone augmentation. Material and Methods The β- TCP scaffold was fabricated by the replica method using polyurethane foam, then coated with PLGA. The PLGA/β- TCP scaffold was characterized by scanning electron miscroscopy ( SEM), transmission electron microscopy ( TEM), X-ray diffraction, compressive testing, cell culture and a subcutaneous implant test. Subsequently, a bone-forming test was performed using 52 rats. The β- TCP scaffold, PLGA-coated scaffold, and β- TCP and PLGA-coated scaffolds loaded with FGF-2, were implanted into rat cranial bone. Histological observations were made at 10 and 35 d postsurgery. Results SEM and TEM observations showed a thin PLGA layer on the β- TCP particles after coating. High porosity (> 90%) of the scaffold was exhibited after PLGA coating, and the compressive strength of the PLGA/β- TCP scaffold was six-fold greater than that of the noncoated scaffold. Good biocompatibility of the PLGA/β- TCP scaffold was found in the culture and implant tests. Histological samples obtained following implantation of PLGA/β- TCP scaffold loaded with FGF-2 showed significant bone augmentation. Conclusion The PLGA coating improved the mechanical strength of β- TCP scaffolds while maintaining high porosity and tissue compatibility. PLGA/β- TCP scaffolds, in combination with FGF-2, are bioeffective for bone augmentation.
- Publication
Journal of Periodontal Research, 2015, Vol 50, Issue 2, p265
- ISSN
0022-3484
- Publication type
Journal Article
- DOI
10.1111/jre.12206