We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
microRNA-210 negatively regulates LPS-induced production of proinflammatory cytokines by targeting NF-κB1 in murine macrophages
- Authors
Qi, Jianni; Qiao, Yu; Wang, Peng; Li, Shuqing; Zhao, Wei; Gao, Chengjiang
- Abstract
Abstract: Ligation of TLR4 with LPS in macrophages leads to the production of proinflammatory cytokines, which are central to eliminate viral and bacterial infection. However, uncontrolled TLR4 activation may contribute to pathogenesis of inflammatory diseases such as septic shock. In this study, we found microRNA-210 was induced in murine macrophages by LPS. Transfection of miR-210 mimics significantly inhibited LPS-induced production of inflammatory cytokines. In contrast, transfection of anti-miR-210 inhibitors increased LPS-induced expression of proinflammatory cytokines. Furthermore, we demonstrated that miR-210 targets NF-κB1. Therefore, our data identify miR-210 as a very important feedback negative regulator for LPS-induced production of proinflammatory cytokines.
- Subjects
BACTERIAL disease treatment; VIRAL disease treatment; MICRORNA; GENETIC regulation; NF-kappa B; INFLAMMATION; CYTOKINES; GENE targeting; LABORATORY mice
- Publication
FEBS Letters, 2012, Vol 586, Issue 8, p1201
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2012.03.011