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- Title
CpG oligodeoxynucleotides discriminately enhance binding capacity of human naïve B cells to Hepatitis B virus epitopes.
- Authors
Bai, Jian-ying; Yang, Yong-tao; Zhu, Rong; Wang, Yi-qin; Tian, Yin; Li, Xiao-huan; Wang, Rong-quan
- Abstract
CpG oligodeoxynucleotides (CpG ODN) have the potential to enhance the antigen-presenting cells function of human naïve B cells. In this study, we aim to define the effect of CpG ODNs on the binding capacity of human naïve B cells for different Hepatitis B virus (HBV) epitopes. Three HLA-A2 restricted epitopes were selected to incubate with CpG ODN-primed human naïve B cells. Binding capacity for each epitope and expression of CD80, CD86, class I major histocompatibility complex (MHC), and class II MHC of naïve B cells was tested, respectively, by flow cytometry. CpG ODNs, especially ODN 2216, enhanced the binding capacity of human naïve B cells for HBV epitopes ( p < 0.01), and induced markedly higher expression of CD80, CD86, class I MHC, and class II MHC. The binding capacity of CpG-treated naive B cells for each epitope was significantly different. In all the 3 subjects, CpG ODN 2216-primed naïve B cells showed the highest binding ability for Env172-180 compared with the other epitopes with a high expression of co-stimulatory and MHC molecules. CpG ODN showed the potential to selectively enhance the binding capacity of human naïve B cells for HBV epitopes. These results suggest new strategies for development of vaccine design.
- Subjects
HEPATITIS B virus; EPITOPES; ANTIGEN presenting cells; MAJOR histocompatibility complex; FLOW cytometry; B cells
- Publication
Canadian Journal of Microbiology, 2012, Vol 58, Issue 6, p752
- ISSN
0008-4166
- Publication type
Article
- DOI
10.1139/w2012-045