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- Title
Chemical-regulated, site-specific DNA excision in transgenic plants.
- Authors
Zuo, Jianru; Niu, Qi-Wen; Møller, Simon Geir; Chua, Nam-Hai
- Abstract
We have developed a chemical-inducible, site-specific DNA excision system in transgenic Arabidopsis plants mediated by the Cre/loxPDNA recombination system. Expression of the Cre recombinase was tightly controlled by an estrogen receptor-based fusion trans-activator XVE. Upon induction by β-estradiol, sequences encoding the selectable marker, Cre, and XVE sandwiched by twoloxP sites were excised from the Arabidopsis genome, leading to activation of the downstream GFP (green fluorescent protein) reporter gene. Genetic and molecular analyses indicated that the system is tightly controlled, showing high-efficiency inducible DNA excision in all 19 transgenic events tested with either single or multiple T-DNA insertions. The system provides a highly reliable method to generate marker-free transgenic plants after transformation through either organogenesis or somatic embryogenesis.
- Subjects
DNA; SURGICAL excision; TRANSGENIC plants
- Publication
Nature Biotechnology, 2001, Vol 19, Issue 2, p157
- ISSN
1087-0156
- Publication type
Article
- DOI
10.1038/84428