We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Effect of NaCl on leaf H<sup>+</sup>-ATPase and the relevance to salt tolerance in two contrasting poplar species.
- Authors
Ma, Xiuying; Deng, Lin; Li, Jinke; Zhou, Xiaoyang; Li, Niya; Zhang, Decai; Lu, Yanjun; Wang, Ruigang; Sun, Jian; Lu, Cunfu; Zheng, Xiaojiang; Fritz, Eberhard; Hüttermann, Aloys; Chen, Shaoliang
- Abstract
During a 30-day period of increasing salinity, we examined the effects of NaCl on leaf H+-ATPase and salinity tolerance in 1-year-old plants of Populus euphratica Oliv. (salt resistant) and P. popularis 35–44 ( P. popularis) (salt sensitive). Electron probe X-ray microanalysis of leaf mesophyll revealed that P. euphratica had a higher ability to retain lower NaCl concentrations in the cytoplasm, as compared to P. popularis. The sustained activity of H+ pumps (by cytochemical staining) in salinised P. euphratica suggests a role in energising salt transport through the plasma membrane (PM) and tonoplast. Salt-induced alterations of leaf respiration, ATP content and expression of PM H+-ATPase were compared between the two species. Results show that P. euphratica retained a constant respiratory rate, ATP production and protein abundance of PM H+-ATPase (by Western blotting) in salt-stressed plants. P. euphratica was able to maintain a comparatively high capacity of ATP hydrolysis and H+ pumping during prolonged salt exposure. By contrast, the activity and expression of PM H+-ATPase were markedly decreased in P. popularis leaves in response to salt stress. Furthermore, NaCl-stressed P. popularis plants showed a marked decline of respiration (70%) and ATP production (66%) on day 30. We conclude that the inability of P. popularis to transport salt to the apoplast and vacuole was partly due to the decreased activity of H+ pumps. As a consequence, cytosolic ion concentrations were observed to be comparatively high for an extended period of time, so that cell metabolism, in particular respiration, was disrupted in P. popularis leaves.
- Subjects
EFFECT of salt on plants; ADENOSINE triphosphatase; HYDROGEN ions; BIOCOMPATIBILITY; POPLARS; PLANT species; SALINITY; LEAVES
- Publication
Trees: Structure & Function, 2010, Vol 24, Issue 4, p597
- ISSN
0931-1890
- Publication type
Article
- DOI
10.1007/s00468-010-0430-0