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- Title
In situ three-dimensional visualization of Bursaphelenchus xylophilus inoculated in Pinus thunbergii using X-ray micro-computed tomography.
- Authors
Nakajima, Gou; Iki, Taiichi; Aikawa, Takuya; Hara, Takumi; Ito, Eisuke; Nakamura, Katsunori
- Abstract
Pine wilt disease, a devastating infectious disease of pine trees, is caused by the pinewood nematode, Bursaphelenchus xylophilus. It is important to understand the spatial dispersal processes of B. xylophilus within host-tree tissues to assess its pathogenic mechanism. However, this is not feasible with conventional microscopy-imaging techniques. In this study, we showed that X-ray micro-computed tomography (micro-CT) imaging can be a powerful tool for visualizing infected nematodes within host-tree tissues. We visualized 161 nematodes and 11 eggs within a Pinus thunbergii stem section, 47.3 mm3 in volume, using an appropriate segmentation of the micro-CT images. Quantitative measurements of the segmented region corresponding to the nematodes allowed for the calculation of the longitudinal length and a-value, which were similar to previous morphological descriptions of B. xylophilus. The technique adopted in this study can aid in understanding the behavior of and obtaining quantitative information on B. xylophilus within tree tissues.
- Subjects
PINEWOOD nematode; THREE-dimensional imaging; CONIFER wilt; PINE; TOMOGRAPHY; X-ray computed microtomography
- Publication
Canadian Journal of Forest Research, 2024, Vol 54, Issue 5, p611
- ISSN
0045-5067
- Publication type
Article
- DOI
10.1139/cjfr-2023-0200