We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Hormone-sensitive lipase-knockout mice maintain high bone density during aging.
- Authors
Wen-Jun Shen; Li-Fen Liu; Patel, Shailja; Kraemer, Fredric B.
- Abstract
We tested the hypothesis that the actions of hormone-sensitive lipase (HSL) affect the microenvironment of the bone marrow and that removal of HSL function by gene deletion maintains high bone mass in aging mice. We compared littermate control/ wild-type (WT) and HSL-/- mice during aging for changes in serum biochemical values, trabecular bone density using micro-computed tomography, bone histomorphometry, and characteristics of primary bone marrow cells and preosteoblasts. There is a regulated expression of HSL and genes involved in lipid metabolism in the bone marrow during aging. HSL-/- mice have increased serum levels of insulin and osteocalcin with decreased leptin levels. Compared with the marked adipocyte infiltration in WT bone marrow (65%/ by area) at 14 mo, HSL-/- mice have fewer (16%, P<0.05) and smaller adipocytes in bone marrow. While/ peak bone density is similar, HSL-/- mice maintain a higher bone density (bone volume/total volume 6.1%) with age than WT mice (2.6%, P<0.05). Primary osteoblasts from HSL-/- mice show increased growth rates and higher osteogenic potential, manifested by increased expression of Runx2 (3.5-fold, P<0.05) and osteocalcin (4-fold, P<0.05). The absence of HSL directs cells within the bone marrow toward osteoblast differentiation and favors the maintenance of bone density with aging.
- Publication
FASEB Journal, 2011, Vol 25, Issue 8, p2722
- ISSN
0892-6638
- Publication type
Article
- DOI
10.1096/fj.11-181016