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- Title
Optimization Extraction of Shikonin Using Ultrasound-Assisted Response Surface Methodology and Antibacterial Studies.
- Authors
Huang, Xiang-Yue; Fu, Hua-Lin; Tang, Hua-Qiao; Yin, Zhong-Qiong; Zhang, Wei; Shu, Gang; Yin, Li-Zi; Zhao, Ling; Yan, Xiao-Rong; Lin, Ju-Chun
- Abstract
The objectives of this study were to develop and optimize ultrasound-assisted extraction (UAE) for shikonin from Arnebia euchroma using response surface methodology (RSM) and to evaluate the antimicrobial activity of shikonin. The maximum yield of shikonin was 1.26% under the optimal extraction conditions (ultrasound power, 93 W; time, 87 min; temperature, 39°C; and liquid-solid ratio, 11 : 1). Shikonin showed inhibitory activity against standard strains and clinical isolates to varying extents (MICs ranging from 128 to 1024 μg/mL, MBCs ranging from 256 to 2048 μg/mL), and it was more effective for Gram-positive bacteria as indicated by lower MIC and MBC values. Time-kill curves revealed that antibacterial activity of shikonin exhibited a dose-response relationship. In summary, via this study, we identified ultrasound-assisted RSM as the optimal extraction method for shikonin, which is a potential material for the treatment of bacterial infections.
- Subjects
BACTERIA; BACTERIAL diseases; DOSE-effect relationship in pharmacology; GRAM-positive bacteria; MICROBIAL sensitivity tests; MOLECULAR structure; QUINONE; TEMPERATURE; TRADITIONAL medicine; ULTRASONICS; PLANT extracts
- Publication
Evidence-based Complementary & Alternative Medicine (eCAM), 2020, p1
- ISSN
1741-427X
- Publication type
Article
- DOI
10.1155/2020/1208617