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- Title
Effect of Huangqin Tang on Urine Metabolic Profile in Rats with Ulcerative Colitis Based on UPLC-Q-Exactive Orbitrap MS.
- Authors
Wang, Dunfang; Ma, Xuran; Guo, Shanshan; Wang, Yanli; Li, Tao; Zou, Dixin; Song, Hongxin; Yang, Weipeng; Ge, Yongxiang
- Abstract
As a classic prescription, Huangqin Tang (HQT) has been widely applied to treat ulcerative colitis (UC), although its pharmacological mechanisms are not clear. In this study, urine metabolomics was first analysed to explore the therapeutic mechanisms of HQT in UC rats induced by TNBS. We identified 28 potential biomarkers affected by HQT that might cause changes in urine metabolism in UC rats, mapped the network of metabolic pathways, and revealed how HQT affects metabolism of UC rats. The results showed that UC affects amino acid metabolism and biosynthesis of unsaturated fatty acids and impairs the tricarboxylic acid cycle (TCA cycle). UC induced inflammatory and gastrointestinal reactions by inhibiting the transport of fatty acids and disrupting amino acid metabolism. HQT plays key roles via regulating the level of biomarkers in the metabolism of amino acids, lipids, and so on, normalizing metabolic disorders. In addition, histopathology and other bioinformatics analysis further confirm that HQT altered UC rat physiology and pathology, ultimately affecting metabolic function of UC rats.
- Subjects
AMINO acids; ANIMAL experimentation; BIOMARKERS; GASTROINTESTINAL system; HERBAL medicine; HIGH performance liquid chromatography; HISTOLOGICAL techniques; INFLAMMATORY mediators; INTESTINAL mucosa; MASS spectrometry; CHINESE medicine; RATS; ULCERATIVE colitis; UNSATURATED fatty acids; BIOINFORMATICS; SULFUR acids; TRICARBOXYLIC acids; METABOLOMICS; PHARMACODYNAMICS
- Publication
Evidence-based Complementary & Alternative Medicine (eCAM), 2020, p1
- ISSN
1741-427X
- Publication type
Article
- DOI
10.1155/2020/1874065