We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Delayed response to cold stress is characterized by successive metabolic shifts culminating in apple fruit peel necrosis.
- Authors
Gapper, Nigel E.; Hertog, Maarten L. A. T. M.; Jinwook Lee; Buchanan, David A.; Leisso, Rachel S.; Zhangjun Fei; Guiqin Qu; Giovannoni, James J.; Johnston, Jason W.; Schaffer, Robert J.; Nicolaï, Bart M.; Mattheis, James P.; Watkins, Christopher B.; Rudell, David R.
- Abstract
Background: Superficial scald is a physiological disorder of apple fruit characterized by sunken, necrotic lesions appearing after prolonged cold storage, although initial injury occurs much earlier in the storage period. To determine the degree to which the transition to cell death is an active process and specific metabolism involved, untargeted metabolic and transcriptomic profiling was used to follow metabolism of peel tissue over 180 d of cold storage. Results: The metabolome and transcriptome of peel destined to develop scald began to diverge from peel where scald was controlled using antioxidant (diphenylamine; DPA) or rendered insensitive to ethylene using 1-methylcyclopropene (1-MCP) beginning between 30 and 60 days of storage. Overall metabolic and transcriptomic shifts, representing multiple pathways and processes, occurred alongside α-farnesene oxidation and, later, methanol production alongside symptom development. Conclusions: Results indicate this form of peel necrosis is a product of an active metabolic transition involving multiple pathways triggered by chilling temperatures at cold storage inception rather than physical injury. Among multiple other pathways, enhanced methanol and methyl ester levels alongside upregulated pectin methylesterases are unique to peel that is developing scald symptoms similar to injury resulting from mechanical stress and herbivory in other plants.
- Subjects
PHYSIOLOGICAL effects of cold temperatures; NECROSIS; CELL death; ANTIOXIDANTS; HERBIVORES; METHYLESTERASES
- Publication
BMC Plant Biology, 2017, Vol 17, p1
- ISSN
1471-2229
- Publication type
Article
- DOI
10.1186/s12870-017-1030-6