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- Title
Evaluating the effects of ellagic acid on pSTAT3, pAKT, and pERK1/2 signaling pathways in prostate cancer PC3 cells.
- Authors
Eskandari, Elaheh; Heidarian, Esfandiar; Amini, Sayed Asadollah; Saffari-Chaleshtori, Javad
- Abstract
<bold>Objective: </bold>One of the most common malignancies among men is prostate cancer. Ellagic acid (EA), a polyphenol antioxidant, has many pharmacological actions, especially anticancer effects. The purpose of this study was to evaluate the effect of EA treatment on interleukin-6 (IL-6) gene expression, cell viability, IL-6 secretion, phosphorylated STAT3, ERK, and AKT cellular signaling proteins in human prostate cancer cells (PC3).<bold>Materials and Methods: </bold>The cytotoxic effects of the EA (0-100 µM) on PC3 cells were determined by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay. IL-6 gene expression was down, using real-time quantitative polymerase chain reaction. The cellular concentration of phosphorylated ERK1/2, AKT, and STAT3 signaling pathways was assessed by Western blotting technic.<bold>Results: </bold>EA treatment of PC3 cells resulted in a reduction of cell viability and phosphorylated STAT3, ERK, and AKT signaling proteins after 72 h in a dose-dependent manner. IL-6 gene expression and IL-6 levels significantly increased (P < 0.05) in a dose-dependent pattern in treated PC3 with EA. Thus, these data suggested the essential role of signaling proteins in EA-mediated anti-proliferation of PC3 cells.<bold>Conclusions: </bold>Our finding shows that EA can be considered as a potent agent that decreases cell proliferation through a reduction of phosphorylated STAT3, ERK, and AKT cellular signaling proteins.
- Subjects
PROSTATE cancer treatment; ELLAGIC acid; GENE expression; CELL survival; ANTINEOPLASTIC agents; PROTEINS; APOPTOSIS; BENZOPYRANS; CARRIER proteins; CELL lines; CELL physiology; CELLULAR signal transduction; PHOSPHORYLATION; PROSTATE tumors; TRANSFERASES; PHARMACODYNAMICS
- Publication
Journal of Cancer Research & Therapeutics, 2016, Vol 12, Issue 4, p1266
- ISSN
0973-1482
- Publication type
journal article
- DOI
10.4103/0973-1482.165873