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- Title
Hunting the Needle in the Haystack: A Guide to Obtain Biologically Meaningful MicroRNA Targets.
- Authors
Karbiener, Michael; Glantschnig, Christina; Scheideler, Marcel
- Abstract
MicroRNAs (miRNAs) are endogenous small non-coding RNAs of ~23 nucleotides in length that form up a novel class of regulatory determinants, with a large set of target mRNAs postulated for every single miRNA. Thousands of miRNAs have been discovered so far, with hundreds of them shown to govern biological processes with impact on disease. However, very little is known about how they specifically interfere with biological pathways and disease mechanisms. To investigate this interaction, the hunt for direct miRNA targets that mediate the miRNA effects--the "needle in the haystack"--is an essential step. In this review we provide a comprehensive workflow of successfully applied methods starting from the identification of putative miRNA-target pairs, followed by validation of direct miRNA-mRNA interactions, and finally presenting methods that dissect the impact of particular miRNA-target pairs on a biological process or disease. This guide allows the way to be paved for obtaining biologically meaningful miRNA targets.
- Subjects
RNA-RNA interactions; RNA interference; MESSENGER RNA; NON-coding RNA; MICRORNA
- Publication
International Journal of Molecular Sciences, 2014, Vol 15, Issue 11, p20266
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms151120266