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- Title
A View of Pre-mRNA Splicing from RNase R Resistant RNAs.
- Authors
Suzuki, Hitoshi; Tsukahara, Toshifumi
- Abstract
During pre-mRNA splicing, exons in the primary transcript are precisely connected to generate an mRNA. Intron lariat RNAs are formed as by-products of this process. In addition, some exonic circular RNAs (circRNAs) may also result from exon skipping as by-products. Lariat RNAs and circRNAs are both RNase R resistant RNAs. RNase R is a strong 3' to 5' exoribonuclease, which efficiently degrades linear RNAs, such as mRNAs and rRNAs; therefore, the circular parts of lariat RNAs and the circRNAs can be segregated from eukaryotic total RNAs by their RNase R resistance. Thus, RNase R resistant RNAs could provide unexplored splicing information not available from mRNAs. Analyses of these RNAs identified repeating splicing phenomena, such as re-splicing of mature mRNAs and nested splicing. Moreover, circRNA might function as microRNA sponges. There is an enormous variety of endogenous circRNAs, which are generally synthesized in cells and tissues.
- Subjects
MESSENGER RNA; EXORIBONUCLEASES; RIBONUCLEASE R; EUKARYOTIC cells; DISEASES -- Management
- Publication
International Journal of Molecular Sciences, 2014, Vol 15, Issue 6, p9331
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms15069331