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- Title
Lectin-Like Bacteriocins from <i>Pseudomonas</i> spp. Utilise D-Rhamnose Containing Lipopolysaccharide as a Cellular Receptor.
- Authors
McCaughey, Laura C.; Grinter, Rhys; Josts, Inokentijs; Roszak, Aleksander W.; Waløen, Kai I.; Cogdell, Richard J.; Milner, Joel; Evans, Tom; Kelly, Sharon; Tucker, Nicholas P.; Byron, Olwyn; Smith, Brian; Walker, Daniel
- Abstract
Lectin-like bacteriocins consist of tandem monocot mannose-binding domains and display a genus-specific killing activity. Here we show that pyocin L1, a novel member of this family from Pseudomonas aeruginosa, targets susceptible strains of this species through recognition of the common polysaccharide antigen (CPA) of P. aeruginosa lipopolysaccharide that is predominantly a homopolymer of d-rhamnose. Structural and biophysical analyses show that recognition of CPA occurs through the C-terminal carbohydrate-binding domain of pyocin L1 and that this interaction is a prerequisite for bactericidal activity. Further to this, we show that the previously described lectin-like bacteriocin putidacin L1 shows a similar carbohydrate-binding specificity, indicating that oligosaccharides containing d-rhamnose and not d-mannose, as was previously thought, are the physiologically relevant ligands for this group of bacteriocins. The widespread inclusion of d-rhamnose in the lipopolysaccharide of members of the genus Pseudomonas explains the unusual genus-specific activity of the lectin-like bacteriocins.
- Subjects
ANTIBACTERIAL agents; BACTERIOCIN genetics; PHYSIOLOGICAL effects of lipopolysaccharides; CELL receptors; PYOCINS
- Publication
PLoS Pathogens, 2014, Vol 10, Issue 2, p1
- ISSN
1553-7366
- Publication type
Article
- DOI
10.1371/journal.ppat.1003898