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- Title
PI3-Kinase-dependent electrogenic intestinal transport of glucose and amino acids.
- Authors
Rexhepaj, Rexhep; Artunc, Ferruh; Metzger, Marco; Skutella, Thomas; Lang, Florian
- Abstract
Intestinal glucose and amino acid transport is stimulated by the serum- and glucocorticoid-inducible kinase isoforms SGK1, SGK2, and SGK3 and protein kinase B which are, in turn, stimulated following activation of the phosphoinositol-3 kinase (PI3 kinase). The present study has been performed to explore whether pharmacological inhibition of the PI3 kinase affects electrogenic jejunal transport of glucose and amino acids. In Ussing chamber experiments, glucose (20 mM), phenylalanine (20 mM), glutamine (20 mM), cysteine (20 mM), and proline (20 mM) generated lumen negative currents ( I glc, I phe, I gln, I cys, and I pro), respectively, which gradually declined following application of the PI3 kinase inhibitor Wortmannin (1 μM). Within 40 min, Wortmannin treatment significantly decreased I glc by 39 ± 10% ( n = 5), I phe by 70 ± 7% ( n = 4), I gln by 69 ± 8% ( n = 4), I cys by 67 ± 8% ( n = 6), and I prol by 79 ± 12% ( n = 3). A similar decline of I glc was observed following application of the PI3 kinase inhibitor LY294002 (50 μM). Exposure to the inhibitors did not significantly alter transepithelial potential difference and resistance in the absence of substrates for electrogenic transport. The observations suggest that the electrogenic transport of glucose and several amino acids requires the continued activity of PI3 kinase.
- Subjects
GLUCOSE; AMINO acids; GLUCOCORTICOIDS; PROTEIN kinases; GASTROINTESTINAL mucosa; ACTIVE biological transport
- Publication
Pflügers Archiv: European Journal of Physiology, 2007, Vol 453, Issue 6, p863
- ISSN
0031-6768
- Publication type
Article
- DOI
10.1007/s00424-006-0154-6