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- Title
Kushenol C Prevents Tert-Butyl Hydroperoxide and Acetaminophen-Induced Liver Injury.
- Authors
Cho, Byoung Ok; Kim, Jang Hoon; Che, Denis Nchang; Kang, Hyun Ju; Shin, Jae Young; Hao, Suping; Park, Ji Hyeon; Wang, Feng; Lee, Yun Ji; Jang, Seon Il; Tesoriere, Luisa
- Abstract
Sophora flavescens, also known as Kushen, has traditionally been used as a herbal medicine. In the present study we evaluated the ameliorative effects of kushenol C (KC) from S. flavescens against tBHP (tert-Butyl hydroperoxide)-induced oxidative stress in hepatocellular carcinoma (HEPG2) cells and acetaminophen (APAP)-induced hepatotoxicity in mice. KC pretreatment protected the HEPG2 cells against oxidative stress by reducing cell death, apoptosis and reactive oxygen species (ROS) generation. KC pretreatment also upregulated pro-caspase 3 and GSH (glutathione) as well as expression of 8-Oxoguanine DNA Glycosylase (OGG1) in the HEPG2 cells. The mechanism of action was partly related by KC's activation of Akt (Protein kinase B (PKB)) and Nrf2 (Nuclear factor (erythroid-derived 2)-like 2) in the HepG2 cells. In in vivo investigations, coadministration of mice with KC and APAP significantly attenuated APAP-induced hepatotoxicity and liver damage, as the serum enzymatic activity of aspartate aminotransferase and alanine aminotransferase, as well as liver lipid peroxidation and cleaved caspase 3 expression, were reduced in APAP-treated mice. Coadministration with KC also up-regulated antioxidant enzyme expression and prevented the production of proinflammatory mediators in APAP-treated mice. Taken together, these results showed that KC treatment has potential as a therapeutic agent against liver injury through the suppression of oxidative stress.
- Subjects
LIVER injuries; PROTEIN kinase B; ASPARTATE aminotransferase; ALANINE aminotransferase; REACTIVE oxygen species; GLUTATHIONE
- Publication
Molecules, 2021, Vol 26, Issue 6, p1635
- ISSN
1420-3049
- Publication type
Article
- DOI
10.3390/molecules26061635