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- Title
Characteristics and complete genome sequence of the virulent Vibrio alginolyticus phage VAP7, isolated in Hainan, China.
- Authors
Gao, Mingming; Qin, Yanhong; Fan, Hang; Zhang, Xianglilan; Li, Puyuan; Liu, Huiying; Pei, Guangqian; Mi, Zhiqiang; Xu, Xuling; Tong, Yigang; Bai, Changqing
- Abstract
A novel Vibrio alginolyticus phage, VAP7, was isolated from seawater collected from Sanya, Hainan province, China. Whole-genome sequencing analysis revealed that phage VAP7 has a linear, double-stranded DNA genome of 144,685 bp with an average G+C content of 41.9% and a high degree of sequence similarity to Vibrio phage VP-1. Annotation results identified 193 open reading frames and one transfer RNA-encoding gene in the phage genome. The morphology and the results of phylogenetic analysis suggest that VAP7 should be classified as a new member of the family Ackermannviridae. Moreover, phage VAP7 grew over a wide pH (5.0-10.0) and temperature (4-40 °C) range. Host-range experiments revealed that VAP7 could infect 31 Vibrio alginolyticus strains. Thus, VAP7 infecting Vibrio alginolyticus strains represents a potential new candidate for use in phage therapy.
- Subjects
HAINAN Sheng (China); VIBRIO alginolyticus; NUCLEOTIDE sequencing; BACTERIOPHAGES; GENETIC transformation; SEQUENCE analysis; VIBRIO
- Publication
Archives of Virology, 2020, Vol 165, Issue 4, p947
- ISSN
0304-8608
- Publication type
Article
- DOI
10.1007/s00705-020-04535-4