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- Title
The involvement of protein kinases and protein phosphatases in signal transduction during neurotransmitter-induced stimulation of root water-pumping activity.
- Authors
Zholkevich, V. N.; Zhukovskaya, N. V.; Popova, M. S.
- Abstract
In detached roots of etiolated maize ( Zea mays L.) seedlings, neurotransmitters, adrenalin and noradrenalin, stimulated exudation by increasing the root pressure due to activation of its metabolic component. In these treatments, the osmotic pressure of the exudate was somewhat reduced. In contrast, a temperature coefficient Q10 was increased, which as in accordance with the increase of the absolute value of the metabolic component and its proportion in the total root pressure. To obtain some information about transmitting the signals induced by adrenalin and noradrenalin action on water transport, we used two inhibitors of the most important and universal elements of signaling pathways, staurosporine (the inhibitor of protein kinases) and okadaic acid (the inhibitor of protein phosphatases). In control roots, staurosporine markedly slowed and okadaic acid accelerated exudation. In the presence of staurosporine in the incubation medium, a stimulatory effect of both neurotransmitters was completely abolished and the rate of exudation became even below the control value. Okadaic acid exerted an opposite action: it augmented markedly stimulatory effects of both neurotrasmitters. The data obtained indicated the involvement of protein kinases and protein phosphatases in transduction of signals induced by adrenalin and noradrenalin, which stimulated root water-pumping activity.
- Subjects
SEEDLINGS; PROTEIN kinases; PHOSPHOPROTEIN phosphatases; GENETIC transduction; ROOT pressure; PLANT roots
- Publication
Russian Journal of Plant Physiology, 2007, Vol 54, Issue 4, p487
- ISSN
1021-4437
- Publication type
Article
- DOI
10.1134/S1021443707040085