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- Title
EVALUATION OF THE ANTIBACTERIAL EFFECT OF DIODE LASER ON STREPTOCOCCUS MUTANS ORAL BIOFILM: A COMPARATIVE STUDY BETWEEN HIGH AND LOW POWERS.
- Authors
Khater, Radwa Yehia; Zaky, Ahmed Abbas; Saber, Hany Mohamed Aly; Hussein, Amira Farouk
- Abstract
Oral Streptococcus is very common in the oral cavity and is considered the most cariogenic pathogen in tooth decay. The purpose of this study is to evaluate the effectiveness of the diode laser using two different powers to control the growth of Streptococcus mutans in the oral biofilm of deciduous teeth in vitro. The power used is 2W (high power) and 200 mW (low power). 48 extracted human primary anterior teeth were prepared and they were equally distributed into four groups: Group I (Negative control), Group II (positive control), Group III (diode laser 980 nm at 2W power) and Group IV (diode laser 980nm at 200 mW power). A biofilm of Streptococcus mutans (ATCC 25175) was prepared and grown on the enamel surface of teeth. The surface topography of the enamel surface of all groups was analyzed using environmental scanning electron microscope (ESEM) Model Quanta 250 FEG (Field Emission Gun) attached with Energy Dispersive X-ray Analyses (EDX) before and after treatment. After the treatment procedures, all the samples were returned to the glass tubes containing 1ml phosphate buffer and incubated for 24 h. After that, the tubes were sonicated to detach the biofilms formed on the enamel surface of samples and the number of Colony Forming Units per milliliter (CFU/ml of dry biofilm weight) in each group was determined. The diode laser (980nm) exhibited a significant antibacterial action against Streptococcus mutans biofilm and reduced its count and viability. There was no significant difference between high and low power diode in reducing bacterial count, although low power diode gave better results. The ESEM results showed that the irradiation with a low power diode laser had a special effect on reducing the bacterial count over the high power diode laser. The calcium and phosphorus enamel content (wt. %) was preserved and increased after diode laser application in both groups. However, there was no significant difference between group III (high power diode) and group IV (low power diode). In conclusion, the diode laser application is a successful method for reducing microbial colonies of streptococcus mutans. There was no significant difference between high and low power diode, although low power diode gave better results.
- Subjects
CARIOGENIC agents; SEMICONDUCTOR lasers; STREPTOCOCCUS mutans; AMELOBLASTS; HIGH power lasers; ENERGY dispersive X-ray spectroscopy; MICROBIAL communities; BIOFILMS
- Publication
Biochemical & Cellular Archives, 2022, Vol 22, Issue 1, p1815
- ISSN
0972-5075
- Publication type
Article