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- Title
IN-VITRO, EVALUATION OF ANTIBACTERIAL ACTIVITIES OF SOME MEDICINAL PLANT EXTRACTS AGAINST METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS (MRSA) ISOLATED AND GENETICALLY IDENTIFIED FROM ACUTE TONSILLITIS.
- Authors
Hussein, Khulood Abdulkareem; Madhkoor, Huda Abdulrahim; Albadran, Reyam Moaid
- Abstract
Methicillin-resistant Staphylococcus aureus (MRSA) is a common infection associated tonsillitis. The aim of this research is to genetically identify MRSA in tonsillitis patients and evaluate herbal medicinal plant extracts against Methicillin-resistant Staphylococcus aureus (MRSA) as multidrug-resistant bacteria, 34 (60.71%). Staphylococcus aureus was isolated from tonsillitis, phenotipcally identified on chrome agar media, and genotyped using the Staph 16S rRNA gene specific for the Staphylococcus aureus and mecA gene for determining Staphylococcus aureus resistance to methicillin (MRSA). The result of amplification of the staph 16S rRNA and mecA genes confirmed that all 34 (60.71%) were Staphylococcus aureus and resistant to methicillin (MRSA). Seven plant extracts, Nigella sativa seeds, Punica granatum peel, Peganum harmala, Eugenia caryophyllus, Cinnamomum zeylanicum, Brassica juncea and Zingiber officinal were tested for antibacterial efficacy against Staphylococcus aureus (MRSA) isolates, as well as testing the cytotoxicity of crude extracts. A well diffusion method was used to complete the primary screening on Staphylococcus aureus (MRSA), both extracts [aqueous and alcoholic] had a broad-spectrum impact, while alcoholic extracts had the greatest effect. However, both extracts had varied effects against all bacterial strain at the minimal inhibitory concentrations. The two extracts had a bactericidal effect, and the growth of all bacteria isolates in this investigation was monitored for seven days with no growth. The cytotoxicity of the extracts on red blood cells (RBCs) was tested, and the results revealed that there was no cytotoxicity in the absence of alcohol. An extract of Cinnamomum zeylanicum enhanced red blood cell volume, swelling, and agglutination. Zingiber officinale and Punica granatum peel alcoholic extract produced the opposite effect. The cells expanded and erupted at concentrations of 200 and 100 of the Peganum harmala alcoholic extract. As a result, the seven plants can be safely employed as a natural bactericidal agent source.
- Subjects
METHICILLIN-resistant staphylococcus aureus; BLACK cumin; PLANT extracts; MEDICINAL plants; HEMATOCRIT; TONSILLITIS
- Publication
Biochemical & Cellular Archives, 2022, Vol 22, Issue 1, p1137
- ISSN
0972-5075
- Publication type
Article