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- Title
Isolation of a Xanthobacter sp. degrading dichloromethane and characterization of the gene involved in the degradation.
- Authors
Emanuelsson, Maria A. E.; Osuna, M. Begoña; Jorge, Ruben M. Ferreira; Castro, Paula M. L.
- Abstract
A bacterial strain able to degrade dichloromethane (DCM) as the sole carbon source was isolated from a wastewater treatment plant receiving domestic and pharmaceutical effluent. 16S rDNA studies revealed the strain to be a Xanthobacter sp. (strain TM1). The new isolated strain when grown aerobically on DCM showed Luong type growth kinetics, with μmax of 0.094 h-1 and Sm of 1,435 mg l-1. Strain TM1 was able to degrade other aromatic and aliphatic halogenated compounds, such as halobenzoates, 2-chloroethanol and dichloroethane. The gene for DCM dehalogenase, which is the key enzyme in DCM degradation, was amplified through PCR reactions. Strain TM1 contains type A DCM dehalogenase (dcmAa), while no product could be obtained for type B dehalogense (dcmAb). The sequence was compared against 12 dcmAa from other DCM degrading strains and 98% or 99% similarity was observed with all other previously isolated DCM dehalogenase genes. This is the first time a Xanthobacter sp. is reported to degrade DCM.
- Subjects
DICHLOROMETHANE; WASTEWATER treatment; INDUSTRIAL waste purification; SEWAGE disposal plants; POLYMERASE chain reaction; HALOCARBONS; ETHYLENE dichloride; ALIPHATIC compounds; AROMATIC compounds
- Publication
Biodegradation, 2009, Vol 20, Issue 2, p235
- ISSN
0923-9820
- Publication type
Article
- DOI
10.1007/s10532-008-9216-0